Figure 7
From: Peroxicretion: a novel secretion pathway in the eukaryotic cell
Secretion of eGFP-SKL mediated by SncA decorated peroxisomes. A. niger strains were cultivated for 24 hours in MEAM. 10 ul samples of the medium were taken and subjected to SDS-PAGE and subsequently to western blotting as indicated by the manufacturer (Invitrogen). Lane 1: positive control eGFP, lane 2: MW marker indicated at the right hand side, lane 3 and 4: eGFP-SKL transformants, lane 5 and 6: eGFP-SKL, SNCA-PMPA co-transformants, lane 7 and 8: untransformed wild type strain.