Figure 6

Expression of full length native and Gene Composer engineered genes in Cell Free System, SDS-PAGE analyses. A, Total protein from CFS expression of B. subtilis FtsZ (42.8 kDa), Hepatitis C Virus NS5B (66.4 kDa); and human P38α (43.5 kDa) were resolved by SDS PAGE and the resulting coomassie blue stained gel is shown. Odd lanes contain expression from native genes, even lanes contain expression from engineered genes. B, Soluble proteins from the total expression were purified by Ni affinity chromatography and resolved by SDS PAGE. Odd lanes contain expression from native genes, even lanes contain expression from engineered genes. Data shown is a representative from three independent experiments.