Figure 2

Effects of different folding additives on the refolding recovery yield. Respective additive was added into the refolding buffer containing 50 mM NaCl, 200 μM NADP⁺, and 10 mM DTT in 50 mM Tris, pH 8.0 in the presence or absence of 400 mM Arg to final concentration of each additive at 40 μM for PEG 3350, 400 mM for Gly, 400 mM for Pro, 400 mM for glucose, 200 mM for (NH₄)₂SO₄, 200 mM for trehalose, 200 mM for AHA, 5% for glycerol, 0.05% for Triton X-100 and 0.05% for butanol. The refolding mixture was incubated at 25°C and the final regained G6PD activity was recorded.