Figure 2
From: RNA degradation compromises the reliability of microRNA expression profiling
Analysis of miRNA degradation by Northern blot analysis. To evaluate how degradation of total RNA affects miRNA integrity the liver specific miR-122 was selected. The top panel shows ethidium bromide stained 5S RNA as a loading control. In the bottom panel background-corrected signal intensities of the northern blot are calculated by ImageJ. The signal corresponding to the miRNA with the highest signal intensity (T0) was set to 100%, and signals for the other samples were calculated as a percentage of it. The position of the mature miR-122 is indicated (middle panel).