Figure 1From: Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryosAnalysis of zfTERT promoter activity in whole zebrafish embryos. Zebrafish one to eight-cell embryos were microinjected with plessEGFPLuc, zfpTERT(1 Kb)-EGFPLuc or zfpTERT(3 Kb)-EGFPLuc and the pRL-CMV (10:1) reporter vectors. Twenty-four hours after microinjection, the firefly and Renilla luciferase activity was measured using the Dual-Luciferase Reporter Assay System. The results are expressed as the mean ± S.E. of normalized luciferase activity relative to plessEGFPLuc injected embryos. *p < 0.05 vs. plessEGFPLuc. †p < 0.05 vs. zfpTERT(1 Kb)-EGFPLuc.Back to article page