Figure 2From: Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtiiGel-electrophoretic analysis of purified, StrepII-tagged Cr HydA1. (A): Coomassie blue staining. (B): Immunoblot detection with Strep tactin AP conjugate. Lanes 1: HydA1 extracted after growth under DMSO-respiring conditions (~1 μg); lanes 2: HydA1 extracted after growth under TMAO-respiring conditions (~1 μg); lanes 3: HydA1 extracted after growth under thiosulfate-respiring conditions (~1 μg). M: molecular weight marker (kDa).Back to article page