Figure 2

Selection of yCD-specific scFvs. IPTG inducted bacterial supernatants of individual colonies from the third round of the ETH-2 selection on yCD protein, were tested by ELISA in 96-well microtiter plates coated with the antigen. OD values higher than three fold the value of negative control are scored as positive. Negative and positive controls positioned in wells 1–4 reacted as expected. In the inserted box, the Western blot of yCD protein detected by scFvH5 (one of the most reactive clones) is shown.