Figure 6

Overexpression of A. gossypii PRS2,4 and PRS3 genes. Top-left, scheme of the modular cassette used for PRS2,4 and PRS3 overexpression. The integration, selection and overexpression modules are indicated. Top-right, Southern blot analysis to confirm correct integration of the overexpression cassettes into AgLEU2 locus. Genomic DNA of the wild-type (lane 1), GPD-PRS2,4 (lane 2) and GPD-PRS3 (lane 3) strains was digested with PstI and a genomic PstI fragment was used as a radioactive probe. Pm, PmeI; S, SalI; N, NdeI; B, BamHI; Ps, PstI. Bottom, northern blot analysis of A. gossypii total RNA (25 μg) obtained from cultures of the wild-type, GPD-PRS2,4 and GPD-PRS3 strains grown in MA2 rich medium. rRNA 28S was used as a loading control.