Figure 4
ELISA on directly immobilized active VEEV particles. Antigen: 1 μg VRS purified VEEV particles or 1 μg VRS concentrated supernatant from non-infected Vero cells as control. A. 1 × 109 scFv phage per well were used for VEEV detection. The scFv phage were detected as described in figure 3. The mean values of two ELISAs from two independent scFv phage productions are shown. B. 10 ng per well (100 ng/mL) scFv-Fc were used for VEEV detection. The scFv-Fc were detected as described in figure 3.