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Figure 5 | BMC Biotechnology

Figure 5

From: Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells

Figure 5

Characterization of non-neural derivatives of NHA cells cultured in expansion medium. Immunocytochemistry results demonstrate silencing of neural markers [GFAP, S100β, MAP2] expression, presence of VIMENTIN, CD44 and abundant expression of FIBRONECTIN (a, b, d) as well as lack of SOX2 (c) in NHA cell derivatives obtained after four passages in Cambrex expansion medium with FBS (differentiation protocol 1).

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