Figure 4From: Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cellsThree derivatives of NHA cells present distinct expression of MAP2, GFAP and CD44. Results of triple immunostainings applied to derivatives of NHA cells obtained according particular differentiation protocols reveal: formation of one phenotypically homogenous [CD44+, MAP2-, GFAP-] non-neural population as a result of protocol 1 (a, b, c, d); presence of neuronal [MAP2+, CD44-, GFAP-] and glial [GFAP+, CD44+, MAP2-] populations in case of protocol 2 (e, f, g, h) and appearance of the third population of non-neural cells [CD44+, MAP2-, GFAP-] coexisting with neuronal [MAP2+, CD44-, GFAP-] and glial [GFAP+, CD44+, MAP2-] lineage in case of protocol 3 (i, j, k, l).Back to article page