Figure 2

Overexpression of 16 putative membrane proteins in the first round of expression screening. After induction at 37°C for 4 hours, cells were harvested and lysed by sonication. The whole cell samples were subjected to SDS-PAGE. The gels were stained by Coomassie blue and the expressions were confirmed by western blot using an antibody against the His-tag. The arrows indicate the expressed proteins for lanes where it is not obvious.