Figure 2
Verification of antibody phage presenting different formats. A Recombinant phages (1013 t.u./lane) were separated on a reducing 12% SDS-PAGE. The gpIII protein was probed using anti-gpIII mAb. The immunoreactive bands were visualized by chemiluminescence substrate detection system. Lane 1, phage-displayed scFv-M6-1B9; lane 2, phage-displayed Fab-M6-1B9 and lane 3, VCSM13 helper phage. Molecular weight markers in kDa are indicated. Arrow indicates recombinant antibody fragment-gpIII fusion proteins (~47 kDa). B CD147-BCCP was captured on wells coated with avidin. Three different concentrations, 1010 – 1012 t.u./ml of phage-displayed scFv-M6-1B9 and phage-displayed Fab-M6-1B9 were added and traced by peroxidase-conjugated anti-M13 phage mAb. VCSM13 helper phage and mAb M6-1B9 specific for CD147 [8] were used as wild-type phage control and antibody control, respectively.