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Figure 10 | BMC Biotechnology

Figure 10

From: Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges

Figure 10

Comparison between DNA/DNA and RNA/DNA mismatch binding affinities. (a) Ranking order of DNA/DNA mismatch binding affinities (extracted from Fig. 4). (b) As anticipated the ranking order for DNA/DNA MMs obtained from the smaller subset of probe sequences (Fig. 9A) is very similar. The ranking order for the analogue RNA/DNA MM duplex stabilities (c) (extracted from Fig. 9B) reveals significant differences in comparison to (b). In part (d) MM-types are ordered according to the hybridization signal differences between RNA/DNA and DNA/DNA MMs (as extracted from Additional file 7). Purine-purine MMs (purine bases highlighted in blue) display the largest decrease of binding afinities with respect to other MM-types.

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BMC Biotechnology

ISSN: 1472-6750

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