Figure 3
From: PCR-based gene synthesis to produce recombinant proteins for crystallization
SDS-PAGE analysis and crystallization of PAZ and PolA. Purified recombinant proteins PAZ and PolA are shown in the upper panels (lanes 2) against standard molecular markers (lanes 1) containing myosin, phosphorylase, BSA, glutamic dehydrogenase, alcohol dehydrogenase, carbonic anhydrase, myoglobin red, lysozyme, aprotinin and insulin B chain on a 11% polyacrylamide gel. Approximately 15 μg of purified protein was loaded in each gel (lanes 2) and visualized with Coomassie brilliant blue. The arrows indicate recombinant proteins at about 15 kDa and 70 kDa for PAZ and PolA respectively. The proteins were estimated to be more than 90% homogeneous. Crystals of PAZ and PolA are shown in the bottom panels along with their corresponding space group and unit cell parameters as determined by preliminary X-ray analysis.