Figure 5
The sketch of metal plate vitrification method and the formation of spherical transparent vitrified droplets. The sketch of metal plate vitrification method shows a metal plate covered with aluminum foil cooled by partial immersion in LN2 contained in a 13 cm width vacuum steel bowl inside the Styrofoam box (A). The droplets, examples of 6 μl and 2 μl transparent vitrified droplets form a spherical shape at temperature of -196°C on the metal surface (distal bar = 3 mm, proximal bar = 2 mm) (B). Crystal transparency of the vitrified droplets (black arrow) is shown in different views. An obscure crystal droplet (white arrow) is shown which may occur after loading preantral follicles is characteristic of damage after thawing.