Figure 3

Affinity purification and cleavage of translated proteins. A. Purification and cleavage of GSTAtMPK6: Molecular weight marker (M), Glutathione-Sepharose coupled kinase (B), AtMPK6 cleaved from the beads (T), AtMPK6 cleaved following elution (E). B. Purification and cleavage of HisAtMPK6: Molecular weight marker (M), MagneHis particle coupled kinase (B), AtMPK6 cleaved from the beads (T), AtMPK6 cleaved following elution (E). Proteins were translated by CEFC and bilayer method, respectively. A quarter of the total amount of purified protein was separated on 12% SDS-PAGE gel and detected by Coomassie Blue staining.