Table 1 FRET model fitting parameters (interchromophore spacing distance and complexation efficiency) for the studied systems.
From: Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET
Donor-acceptor distance | Complexation efficiency (C.E) = (1 - γ) × 100 | ||||
|---|---|---|---|---|---|
Lipoplex Preparation/incubation | (+/-) | d (Å) | R0 (Å) | (+/-) | C.E (%) |
PBS | 14–18 | 38 | 43.0 | 14 | 100 |
10 | 97 | ||||
5 | 96 | ||||
1 | 23 | ||||
Tris-HCl | 5–18 | 40 | 42.0 | 5 | 100 |
1 | 60 | ||||
0.5 | 25 | ||||
Tris-HCl/PBS | 10–18 | 35 | 43.0 | 10 | 100 |
5 | 95 | ||||
3 | 90 | ||||
1 | 53 | ||||
0.5 | 23 | ||||
Tris-HCl/PBS+FBS | 14–18 | 30 | 42.0 | 14 | 100 |
10 | 94 | ||||
5 | 94 | ||||
3 | 89 | ||||
1 | 55 | ||||
0.5 | 15 | ||||