Table 4 Characterization of the recombinant Bm86, Bd86 and Ba86 purification process.
Purification stages | Bm86 | Bd86 | Ba86 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Total protein conc. (mg·L-1) | Rec. protein conc. (mg·L-1) | Purity (%) | Recovery (%) | Total protein conc. (mg·L-1) | Rec. protein conc. (mg·L-1) | Purity (%) | Recovery (%) | Total protein conc. (mg·L-1) | Rec. protein conc. (mg·L-1) | Purity (%) | Recovery (%) | |
Fermentation supernatant | 274 | 150 | 55 | --- | 194 | 110 | 57 | --- | 170 | 112 | 66 | --- |
Culture separation and microfiltration | 137 | 96 | 70 | 60 | 84 | 63 | 75 | 55 | 99 | 77 | 78 | 62 |
Ultrafiltration and diafiltration | 407 | 326 | 80 | 35 | 451 | 370 | 82 | 42 | 370 | 314 | 85 | 40 |