Figure 4
From: Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter
Comparison of PRL-induced expression obtained from the pGL4-CISH versus other PRL-responsive reporters in T47D transfectants in the presence and absence of overexpressed Stat5. T47D cells were transfected with vectors and PRL-stimulated as described above. A. The basal luciferase expression of the various PRL-responsive reporters in T47D transfectants in the absence of PRL. B. The response of reporters to PRL in the presence or absence of overexpressed Stat5a in T47D transfectants. C. The low inset is Western blot demonstrating Stat5a overexpression level obtained with the various transfectants with the arrows indicating each lane of treatments. D. Level of transfected Stat5a influences pGL4-CISH expression. Varying concentrations as indicated of Stat5a expression construct were simultaneously transfected with the pGL4-CISH reporter. Error bars represent SEM; * denotes P < 0.05, and *** denotes P < 0.001 as compared no PRL stimulation alone.