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Table 3 Eight of TaqMan® Gene Expression Assays. Seven of these assays were inventoried by Applied Biosystems (P/N: 4331182, Applied Biosystems, CA, USA) and one was a designed assay (GAPDH-67 Forward primer: CAT CCA TGA CAA CTT TGG TAT CGT; Reverse primer: GGG TGG CAG TGA TGG CAT; Probe: ACT CAT GAC CAC AGT CC).

From: Improved RNA quality and TaqMan®Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials

Gene Symbol – Amplicon Length

Gene Name

Assay ID

MT4 – 62

metallothionein IV

Hs00262914_m1

GAPDH – 67

glyceraldehyde-3-phosphate dehydrogenase

Designed

CDKN1B – 71

cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Hs00153277_m1

MAPK4 – 72

mitogen-activated protein kinase 4

Hs00177074_m1

CD44 – 86

CD44 molecule (Indian blood group)

Hs00153304_m1

SDC2 – 103

syndecan 2 (heparan sulfate proteoglycan 1, cell surface-associated, fibroglycan)

Hs00299807_m1

GAPDH – 122

glyceraldehyde-3-phosphate dehydrogenase

Hs99999905_m1

HLA-A – 164

major histocompatibility complex, class I, A

Hs00740413_g1