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Table 1 Effects of LOWESS Normalization on Correlations.

From: Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements

Effects of LOWESS Normalization

 

Scan Interval (minutes):

0

13

26

39

52

65

78

91

Non- carbon-filtered

Raw ratio data

1.00

0.93

0.81

0.69

0.59

0.48

0.40

0.37

 

Normalized ratio data

1.00

0.98

0.96

0.94

0.92

0.88

0.84

0.80

 

Cy5 Intensity data

1.00

0.98

0.96

0.94

0.90

0.85

0.80

0.76

 

Cy3 Intensity data

1.00

0.99

0.99

0.99

0.98

0.98

0.98

0.97

Carbon-filtered

Raw ratio data

1.00

0.99

0.99

0.99

0.98

0.98

0.97

0.96

 

Normalized ratio data

1.00

0.99

0.99

0.99

0.98

0.98

0.98

0.98

 

Cy5 Intensity data

1.00

0.99

0.99

0.99

0.99

0.99

0.99

0.99

 

Cy3 Intensity data

1.00

0.99

0.99

0.99

0.99

0.99

0.99

0.99

  1. Intensity data and Cy5/Cy3 ratio (raw) data were obtained for individual microarray features at each scan time using the Axon GenePix Pro software. LOWESS normalization was performed on the ratio data. The data shown are correlations (r-values) of the raw or normalized data at each scan interval compared to the 0-minute scan interval.