Table 1 Primers used in RT-PCR and generated PCR product sizes (in base pairs). During PCR the tag used as antisense primer was CAACAGACGCACGACGCAGCAGAC (bold in the tagged RT primer sequences).
From: Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes
Gene | Tagged RT primer | Sense primer | PCR product |
|---|---|---|---|
hupS | CAACAGACGCACGACGCAGCAGACGGTAATTCTTTAGGAACTC | CCATGTCACCCAACCCCAGCGAATCAG | 533 bp |
rnpB | CAACAGACGCACGACGCAGCAGACGAAAGAGCAGTACATAAGC | AGGGTGCAAAGGTGCGGTAAGAGCG | 232 bp |
rbcL | CAACAGACGCACGACGCAGCAGACGAAACGGATATCTTCTAGAC | CGTTCCGCATGACACCCCAGCC | 329 bp |