Figure 4
From: Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes
Agarose gel separation of PCR products. Lanes M – molecular weight markers (GeneRuler 100 bp DNA Ladder, Fermentas). PCR reactions using tags and sense primers (see Table 1) for three Nostoc PCC73102 genes – uptake hydrogenase small subunit (hupS), ribonuclease P subunit B (rnpB) and rubisco large subunit (rbcL). Reactions were run containing either 100 ng of genomic DNA (gDNA) or complementary DNA (cDNA) as template.