Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog

Table 4 ELISA results for HEK-293 ACRP30 cells with and without gene inserts. The results shown are from 1 of 3 different runs performed, but are representative of all 3 runs

Cell line	Viable cell density (10⁶ cells/mL)	Total protein production (ng)	Average specific protein production ( $\frac{ng \cdot mL}{cells}$ )
control with plasmid	1.24 ± 0.01	1580 ± 130	1.27 × 10^-3
+ cdkl3	1.40 ± 0.02	1900 ± 160	1.36 × 10^-3
+ cox15	1.31 ± 0.03	1750 ± 190	1.34 × 10^-3

Note: Each cDNA slide analyzed had at least 3 spots for each of the genes listed above. Analysis of 4 slides translated to a total of at least 12 ratios for each gene. Both the maximum and the minimum expression ratios per gene were determined from these 12 ratios (i.e. sample size).

ISSN: 1472-6750