A systematic approach for testing expression of human full-length proteins in cell-free expression systems

BMC Biotechnology

Table 2 Proteins not expressing in E. coli in vitro or in wheat germ or in both systems. Molecular weight was calculated by translation of the DNA sequence. Localization information was taken from the Uniprot database. Domain information was retrieved from the Pfam database: cc: coiled coil, tms: transmembrane segment, sp signal peptide. Empty fields correspond to no assignment in the database.

Hit Acc. No.	Gene symbol	Mw in kDa	Localization	Domains	Expression in E. coli in vitro	Expression in wheat germ
NM_007317	KIF22	74	nuclear	1cc,,	no	no
NM_015723	PNPLA8	89	membrane		no	no
NM_020748	KIAA1287	135	n/a		no	no
NM_018127	ELAC2	93	nuclear		no	no
NM_015072	TTLL5	92	n/a	3cc,,	no	no
NM_014585	SLC40A1	63	membrane	,,10tms	no	no
NM_020470	YIF1A	32	membrane	,,5tms	no	yes
NM_004394	DAP	12	secreted		no	yes
NM_001014835	PAK4	64	n/a		no	yes
NM_013296	GPSM2	55	n/a		no	yes
NM_006812	OS-9	70	n/a	1cc1sp1tms	no	yes
NM_014860	SUPT7L	47	n/a	1cc,,	no	yes
NM_003908	EIF2S2	39	nuclear		no	yes
NM_006321	ARIH2	58	nucleus	2cc,,	no	yes
NM_002816	PSMD12	53	cytosol	1cc,,	no	yes
NM_000984	RPL23A	18	cytosol		no	yes
NM_002134	HMOX2	36	microsomal	1cc,1tms	yes	no
NM_018099	MLSTD1	60	intracellular	,,2tms	yes	no
NM_013277	RACGAP1	71	intracellular	1cc,,	yes	no
NM_003263	TLR1	91	membrane	1sp,1tms	yes	no
NM_032292	FLJ20203	91	n/a	1cc,,	yes	no
NM_014149	HSPC049	78	n/a	1cc,,	yes	no

ISSN: 1472-6750