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Table 1 Primers and probe used in this study

From: Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilacticiUVA1

Primer/probe

Target/specificity

Sequence (5'-3')

Product size (bp)

Reference

Bak11w

16S rDNA

AGT TTG ATC MTG GCT CAG

 

[35]

Bak4

 

AGG AGG TGA TCC ARC CGC A

 

[36]

P1

pedA-pedB

AAA ATA TCT AAC TAA TAC TTG

  

P2

 

TAA AAA GAT ATT TGA CCA AAA

711

[23]

pedopF

pedB-pedD

GGG CGA GTT TAA CAT GCT AGA

  

pedopR

 

TGA TTA TGA ATT AAC CGT GCA

2862

This study

pedseqA

Sequencing of the pediocin operon

CTT GCT CGA TAA TGG TAA

  

pedseqB

 

CTA TCA GGT AAC TGA AAA

  

pedseqC

 

CAC GCT TTT CTG ATG CAA

  

pedseqD

 

TTC TTG ACC CCA TTA GAA

 

This study

pedseqL

Upstream of operon

AGC AAT TAC AGT CAA CCA TAA CCA T

  

pedseqH

 

ACA GAG CAG GAA TGT TTG CCA CAA G

1009

This study

pedseq M

Downstream of operon

ATT GTC TCC GGC ACA ATG TT

  

pedseqN

 

CAA AGT GGG GAA ACT CGA AA

1417

This study

pedA2RTF

Real-time PCR

GGC CAA TAT CAT TGG TGG TA

  

pedA2RTR

 

ATT GAT TAT GCA AGT GGT AGC C

  

TqM-pedA

 

FAM-ACT TGT GGC AAA CAT TCC TGC TCT GTT GA-TAMRA

100

This study