New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylase

BMC Biotechnology

Table 4 Immobilization of acylases

Enzymea	U_PGK^bloaded	Protein loaded (mg/g of support)	% of protein immobilized	Activity (U_PGK^b/g of support)	Specific activity (U_PGK/mg of immobilized enzyme)^c
PGA wt	100	1.8	97	45.2	26.5 ± (0.5)
PGA-αE194K	100	2.8	100	63.4	22.0 ± (0.7)
PGA-βD494K	138	3.0	95	73.2	24.4 ± (0.4)
PGA-βD403K	100	2.2	97	54.6	26.0 ± (0.6)
PGA-2K	139	3.0	97	86.2	28.7 ± (0.8)
PGA-3K	80	3.2	97	54.0	17.4 ± (0.2)

^aThe enzymes were immobilized on agarose beads activated with aldehyde groups at pH 10 and 4°C.
^bU, μmol of PGK min^-1 mg^-1.
^cActivities of immobilized enzymes are expressed as units (μmol of PGK hydrolyzed min^-1 mg^-1) per gram of agarose beads.

ISSN: 1472-6750