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Table 3 Specific activities of the purified acylases

From: New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylase

Purified enzyme

UaNIPAB/mg

UaPGK/mg

PGA wt

18.5

54.0

PGA-αE194K

14.0

40.0

PGA-βD494K

21.0

46.6

PGA-βD403K

12.8

46.3

PGA-2K

16.4

45.0

PGA-3K

14.0

25.0

  1. aU, μmol of substrate hydrolyzed min-1 mg-1.