Table 2 Activities of the new mutants obtained by single or multiple combinations of aminoacid substitutions
From: New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylase
N. of Lys introduced | Mutants | Expression of the 95 kDa pre-pro-enzyme | Enzyme processinga | U(NIPAB)/mlb | U(NIPAB)/mg |
|---|---|---|---|---|---|
0 | Wt | + | + | 25.0 | 5.0 |
1 | αE194K | + | + | 23.2 | 5.3 |
|  | βD494K | + | + | 27.0 | 5.4 |
|  | βD403K | + | + | 13.9 | 4.6 |
|  | βR495K | + | - | n.d.c | n.d.c |
|  | βD216K | + | - | n.d.c | n.d.c |
2 | αE194K, βD494K | + | + | 20.0 | 4.0 |
|  | βD216K, βG219K | + | - | n.d.c | n.d.c |
|  | αN202K, αN205K | - | - | n.d.c | n.d.c |
3 | βD13K, βE272K, βR276K (3K) | + | + | 19.0 | 4.3 |
4 | (3K)+ βD494K | + | - | n.d.c | n.dc. |
|  | (3K)+ βR495K | + | - | n.d.c | n.d.c |
5 | 3K+ βD216K+βG219K | + | - | n.d.c | n.d.c |