Table 1 Forward and reverse primers used for the PCR mutagenesis

Name

Sequence

D216K rev

5'-CAAAAGGCAATAGCCCTTTCCACTTCCATTTTCCCGTACC-3'

E194K for

5'-CCACTATTGCCGTACAAAAGAGTAACTACCCAC-3'

E194K rev

5'-GTGGGTAGTTACTCTTTTGTACGGCAATAGTGG-3'

D403K for

5'-AGGCGGTGCAGGGAAAGAAATCACCAATCCC-3'

D403K rev

5'-GGGATTGGTGATTTCTTTCCCTGCACCGCCT-3'

D494K for

5'-CCAACGACAAGCAAACGTCCTGTGCTTGCC-3'

D494K rev

5'-GGCAAGCACAGGACGTTTGCTTGTCGTTGG-3'

R495K for

5'-CAACGACAAGCGATAAGCCTGTGCTTGCC-3'

R495K rev

5'-GGCAAGCACAGGCTTATCGCTTGTCGTTGG-3'

D216K/G219K for

5'-ACGGGAAAATGGAAGTGGAAAAAGCTATTGCCT-3'

D216K/G219K rev

5'-AGGCAATAGCTTTTTCCACTTCCATTTTCCCGT-3'

N202K/N205K for

5'-CCCACTTAAATTTAAGCAGCAAAAGTCGCAAACAGC-3'

N202K/N205K rev

5'-GCTGTTTGCGACTTTTGCTGCTTAAATTTAAGTGGG-3'