Figure 5From: New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylasevs/vh1 ratio in the acylation reaction of β-lactam nuclei. vs/vh1 ratio for the N-acylation of 7-ACA with MAME was evaluated at pH 6.5 and 4°C (5 mM). The ratio of all the glyoxyl agarose (violet column) derivatives, which were obtained with the native and mutated acylases, was compared to that of the soluble enzymes (light blue column).Back to article page