Figure 2From: New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylaseSDS-PAGE analysis of the purified native and mutated acylases. Arrows indicate the α (24 KDa) and β subunit (65 Kda) of the mature enzymes. Lane 1: MW marker; Lane 2: PGA wt; Lane 3: PGA-αE194K; Lane 4: PGA-βD494K; Lane 5: PGA-βD403K; Lane 6: PGA-2K (PGA-αE194K-βD494K); Lane 7: PGA-3K (PGA-βD13K-E272K-R276K).Back to article page