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Figure 10 | BMC Biotechnology

Figure 10

From: Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac

Figure 10

The cis -activating function of the 3'-TRD internal domain sequence is essentially orientation-independent. The genetic structures of the different piggyBac vectors assayed in this experiment (A-G) are shown above the plot. The dual constitutive (ie1-hr5-ie1) transcriptional control element is indicated by open boxes, the dual inducible (P CMVmin -TetO7-P CMVmin ) transcriptional control element is indicated by horizontally striped boxes, the orientations of the various heterologous genes are shown, and the new 3'-TRD internal domain sequence introduced into the 5'-TRD region is marked with an cross-hatched oval and an arrow to show its orientation. The plot shows the relative levels of GalT activity induced by each of the indicated piggyBac vectors, together with the background levels determined using extracts of mock-transfected Sf9 cells (None).

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BMC Biotechnology

ISSN: 1472-6750

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