Figure 1
From: Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli
Design of E. coli host cells for the simultaneous overproduction of recombinant target proteins and major chaperone systems. Host cells for recombinant protein expression containing combinations (1 to 8) of two or three chaperone expression plasmids. Each combination of plasmids includes one plasmid carrying the lacIQgene to allow for IPTG controlled chaperone expression.