Table 3 Strains and plasmids used. KmR, CmR and ApR are kanamycin, chloramphenicol and ampicillin resistance, respectively.
Strains and plasmids | Genotype | Source |
|---|---|---|
Strains | ||
E. coli TG1 | supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK - mK -) [F' traD36 proAB lacIqZΔM15] | [51] |
E. coli MC4100 | F- araD139 ΔlacU169 rpsL thi fla | [52] |
E. coli HD705 | MC4100 ΔhycE; defective in large subunit of the hydrogenase 3 subunit | [14] |
E. coli SE1174 | thi-1 leu-6 suc-10 bioA2? galT27 rpsL129 chlC3 λ- fhlA102::Tn10; defective transcription of the hyc operon and fdhF | [53] |
E. coli SE1497 | cysC43 srl-300::Tn10 thr-1 leu-6 thi-1 proA2 galK2 ara-14 xyl-5 mtl-1 lacY1 his-4 argE3 rpsL31 tsx-33 Δ(srl-fhlA); large deletion in the 59 min region removes the hyc, hyp or fhlA genes | [54] |
E. coli PMD23 | MC4100 ΔhypF ; defective in full maturation of hydrogenase | K. T. Shanmugam |
E. coli WL400 | selD (CmR) | [55] |
E. coli BW25113 | lacI q rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78 | Yale CGSG Stock Center |
E. coli BW25113 ΔhyaA Δkan | E. coli JW0954 [41] Δkan; defective in small subunit of hydrogenase 1 | this study |
E. coli BW25113 ΔhyaB Δkan | E. coli JW0955 [41]Δkan; defective in large subunit of hydrogenase 1 | this study |
E. coli BW25113 ΔhybB Δkan | E. coli JW5494 [41] Δkan; defective in probable cytochrome Ni/Fe component of hydrogenase 2 | this study |
E. coli BW25113 ΔhybC Δkan | E. coli JW2962 [41]Δkan; defective in probable large subunit of hydrogenase 2 | this study |
E. coli BW25113 ΔhycE Δkan | E. coli JW2691 [41] Δkan; defective in large subunit of hydrogenase 3 | this study |
E. coli BW25113 ΔhycG Δkan | E. coli JW2689 [41]Δkan; defective in subunit of hydrogenase 3 and formate hydrogenlyase complex | this study |
E. coli MW1000 | BW25113 ΔhyaB ΔhybC Δkan; defective in large subunit of hydrogenase 1 and 2 | this study |
E. coli MW1001 | BW25113 ΔhyaB ΔhybC ΔhycE Δkan; defective in large subunit of hydrogenases 1, 2, and 3 | this study |
E. aerogenes HU-101 | Hydrogen-producing bacteria (positive control strain; wild type) | NBRC |
Plasmids | ||
pBS(Kan)Synhox | pBS(Kan) plac::hoxEFUYH; KmR, expresses hydrogenase genes derived from Synechocystis sp. PCC 6803 | this study |
pBS(Kan)HoxU | pBS(Kan) plac::hoxU; KmR, expresses HoxU derived from Synechocystis sp. PCC 6803 | this study |
pBS(Kan)HoxEFU | pBS(Kan) plac::hoxEFU; KmR, expresses HoxEFU derived from Synechocystis sp. PCC 6803 | this study |
pBS(Kan)HoxFU | pBS(Kan) plac::hoxFU; KmR, expresses HoxFU derived from Synechocystis sp. PCC 6803 | this study |
pBS(Kan)HoxFUY | pBS(Kan) plac::hoxFUY; KmR, expresses HoxFUY derived from Synechocystis sp. PCC 6803 | this study |
pBS(Kan)HoxUYH | pBS(Kan) plac::hoxUYH; KmR, expresses HoxUYH derived from Synechocystis sp. PCC 6803 | this study |
pCP20 | ApR and CmR plasmid with temperature-sensitive replication and thermal induction of FLP synthesis | [43] |