Figure 9From: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomesImmunostained viral plaques of recombinant viruses derived by the BAC allele replacement technique. Virus was isolated following transfection of Vero cells with recombinant HSV2-BACs expressing A33R or B5R, titered and used to infect plates of Vero cells. Monolayers were fixed and then probed with mouse anti-HA monoclonal antibody, followed by AP-conjugated goat anti-mouse antibody. (A) B5R-expressing HSV2-BAC virus infected cells. (B) A33R-expressing HSV2-BAC virus infected cells.Back to article page