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Figure 4 | BMC Biotechnology

Figure 4

From: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes

Figure 4

Construction of an HSV-2 BAC containing att R lambda recombination sites. (A) Schematic diagram of allele replacement vector pKO5.2-UL41-attR-EGFP containing UL41 sequences flanking the attR sites on either side of the EGFP gene cassette. (B) Schematic diagram of DEST-BAC, and HSV2-BAC containing the EGFP gene flanked by lambda phage attR sites for targeting gene replacements into UL41.

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BMC Biotechnology

ISSN: 1472-6750

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