Figure 5From: A novel expression system of domain I of human beta2 glycoprotein I in Escherichia coliDI purification using nickel chromatography. Samples analysed by 15% SDS-PAGE. Lane 1, protein molecular weight marker; lane 2, periplasmic extract; lane 3, flow-through from nickel column; lane 4, wash with 5 mM imidazole; lane 5, wash with 45 mM imidazole; lane 6, elution with 300 mM imidazole.Back to article page