Figure 4
From: Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals
Importance of U1 snRNP in the activity of the 5' splice site-containing tail. A uniformly labeled Bcl-x pre-mRNA was incubated for 2 hours in HeLa extracts treated with 0.15 μM of 2'-O-Me oligonucleotides complementary to the 5' end of U1 snRNA. A mock-treated extract was used as control. Increased amounts of the bifunctional oligonucleotide M4-25D were tested (7.4, 37 and 74 nM). Splicing products were fractionated in a 6% denaturing polyacrylamide gel. The position of the lariat splicing products is shown.