Figure 2
From: A simple vector system to improve performance and utilisation of recombinant antibodies
A comparison of scFv expression using auto-induction media and IPTG induction. A: ScFv expressed from pSANG10-3F; B: ScFv-AP expressed from pSANG14-3F. BL21 (DE3) were grown overnight at 30°C using either auto-induction media (Auto-Ind) or mid-log phase induction with IPTG. The bacterial cell pellet was lysed and analysed by western-blot (10 μl/lane) using an anti-histidine tag mouse monoclonal antibody and Cy5 labelled anti-mouse detection system.