|
|---|
Constructs | % GFP positive cells | Peak Value |
|---|
293T | 0.05 | 1 |
pMigR | 20.78 | 200 |
pMigR-Hd | 15.8 | 14 |
pMigR-ATG | 26.85 | 234 |
pMigR-Hd-BAZF | 17.53 | 18 |
pMigR-ATG-BAZF | 25.33 | 223 |
- Top: Comparison of sequences flanking the initiation codon in high (Wild type IRES) and low (HindIII-modified IRES) IRES activity bicistronic vectors.
- Bold font represent the original ATG EMCV IRES initiation codon, and the corresponding modified HindIII site. Italic font is part of the NcoI site (position 870 in the EMCV sequence) that can be used to clone the cDNA of interest under IRES control.
- Bottom: FACS analysis of GFP expression of the different vectors.
- HEK 293T cells were transfected with the indicated constructs, and subjected to FACS analysis 2 days post-transfection. The percentages of detected GFP positive cells are indicated, as well as the peak value of the GFP signals.
