Figure 2
From: Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR
Agarose gel separation of PCR products. Lanes M – molecular weight markers (GeneRuler 100 bp DNA Ladder, Fermentas). Lanes 1 to 6 – PCR reactions using tags for priming and genomic DNA as template (see Table 1). Lane 7 – PCR positive control using a primer pair for ftsZ and Nostoc PCC73102 genomic DNA as template. Lanes 8 to 13 – PCR reactions using genome specific primers and genomic DNA as template (see Table 1). Lane 14 – PCR negative control using a primer pair for Nostoc PCC73102 ftsZ gene.