Figure 5From: In planta production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus XPurified virions are immunogenic. Staining with Comassie Brilliant Blue (A) of mock-inoculated control plants (A1), partially purified viral particles of the PVX6HisE212ACP (A2) and PVX6His2ACP (A3). Western blotting with anti-6His (B upper) and anti-CP (B lower) antibodies of partially purified viral particles of the PVX6HisE212ACP (B1) and PVX6His2ACP (B2). Western blotting analysis (C) using sera from rabbit inoculated with PVX6His2ACP against proteins purified from mock-inoculated control plants (C1) and from PVX6His2ACP (C2). Western blotting analysis (D) using sera from rabbit inoculated with PVX6HisE212ACP. Purified E2 protein (D1; E2 control protein is indicated with a bold arrow), marker (D2), proteins purified from plants inoculated with PVX6HisE212ACP (D3), mock-inoculated control plants (D4) and PVX6His2ACP (D5). Standards Molecular weight (kDa = kiloDaltons) are shown on the left of each figure.Back to article page