Figure 4From: Generation of human antibody fragments against Streptococcus mutansusing a phage display chain shuffling approachBinding of human diabodies to SAI/II and S. mutans coated ELISA plates. One μg SAI/II or 10 ml S. mutans culture were immobilized on the ELISA plate. Binding of 1 μg human diabody B10, D12 and H6 was detected via the His6tag. The experiments were carried out in duplicate and the mean values are shown. The three human diabodies recognize the SAI/II antigen in the purified form as well as in the context of the bacterial surface.Back to article page