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Table 2 Germline origin of all the sequences.

From: Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor

Clone

  

Germline V sequences

 

EMBL Accession number

Heavy Chain (VH)

Light Chain (VL)

Clone GLE4

AJ704539

1-02 (Z14071)

L11 (X93621)

Clone GL2D

AJ704837

**

7a (Z73659)

Clone GLB10

AJ704836

4-30.2 (Z12364)

1e (Z22193)

Clone GL5D

AJ704835

4-59 (Z12371)

9a (Z73675)

Clone GL D6

AJ704834

1-12 (M99638)

1a (Z73653)

Clone GL B6

AJ704538

6-01 (Z12374)

O11/O1 (X93631)

Clone GL A5

AJ704537

1-02 (Z14071)

1g (Z73663)

Clone GL C4

AJ704536

1-02 (Z14071)

1e (Z73656)

Clone GL D5

AJ704535

4-b (M12071)

L12 (X72813)

Clone GL G11

AJ704532

4-30.4 (Z14074)

1g (Z73663)

Clone GL A8

AJ704533

1-02 (Z14071)

1g (Z73663)

Clone GL A12

AJ704534

4-61 (Z12366)

L12 (X72813)

  1. **Insert size of the clone GL2D is 750 bp and there are segments missing in the heavy chain, making it difficult to attribute a matching germline sequence. However, the remaining portions of the heavy chain confirm its identity as part of heavy chain.
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BMC Biotechnology

ISSN: 1472-6750

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