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Table 1 Transforming units (TUs) of PLAP binding phage. Transforming units (TUs) of PLAP binding phage after each round of selection. There was an initial fall in the TUs of the eluted phage in comparison to the input phage. There was also a decrease in the TUs of the phage eluted after the second round of selection indicating the decrease in phage binding non-specifically to the beads. For the rounds one and two, the selection was carried out on magnetic beads. For the rounds three and four, the selection was carried out on immunotubes.

From: Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor

Rounds of selection

Transforming units (input)

Transforming units eluted with pNPP (output)

Transforming units eluted with L-Phe-gly-gly + pNPP (output)

Unselected library

1014

-

-

First

1014

102

1012

Second (PLAP- conjugated beads were reduced)

1014

THIS STEP WAS NOT PERFORMED FOR THE SECOND ROUND OF SELECTION

109

Third (selection on Immunotubes)

1014

106

104

Fourth (selection on Immunotubes)

1014

107

106