Rounds of selection | Transforming units (input) | Transforming units eluted with pNPP (output) | Transforming units eluted with L-Phe-gly-gly + pNPP (output) |
---|---|---|---|
Unselected library | 1014 | - | - |
First | 1014 | 102 | 1012 |
Second (PLAP- conjugated beads were reduced) | 1014 | THIS STEP WAS NOT PERFORMED FOR THE SECOND ROUND OF SELECTION | 109 |
Third (selection on Immunotubes) | 1014 | 106 | 104 |
Fourth (selection on Immunotubes) | 1014 | 107 | 106 |