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Figure 9 | BMC Biotechnology

Figure 9

From: Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor

Figure 9

Cell ELISA to study the binding of the isozyme specific scFvs to the cell surface expressed PLAP.The two PLAP-specific purified scFvs, clone E4 and clone GL2D showed significant binding to the HeLa cells that express PLAP (solid bars). SaOs-2 cells, that do not express PLAP, served as control (empty bars). The scFv VB1 did not show binding to either of the cell types and served as a negative scFv control. The black bars on top indicate SD.

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BMC Biotechnology

ISSN: 1472-6750

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