Figure 8From: Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitorLineweaver-Burk Plot of inhibition of PLAP activity by purified GLD6.The enzyme activity of PLAP is inhibited in a competitive manner. Km of the enzyme: 5.5 mM; Km of the enzyme with GLD6: 15 mM. Inset shows % inhibition of enzyme activity by varying amount of purified scFv GLD6 (This clone has a higher binding [by ELISA] to PLAP and BAP as compared to IAP).Back to article page