Figure 7From: Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitorLineweaver-Burk Plot of inhibition of PLAP activity by purified GL2D.The enzyme activity of PLAP is inhibited in a competitive manner. Km of the enzyme: 5.5 mM; Km of the enzyme with GL2D: 25 mM. Inset shows % inhibition of enzyme activity by varying amount of purified scFv GL2D (This clone also shows a higher binding [by ELISA] to PLAP as compared to BAP or IAP).Back to article page